LABORATOR  INFECTII ENTERICE VIRALE

Sef laborator: Dr. Anda Baicus, medic primar, doctor in stiinte, sef de lucrari,  CSIII, abaicus@cantacuzino.ro

Personal laborator:    

Dr. Ana Ioana Persu, medic primar, doctor in stiinte, polerad@cantacuzino.ro 

Cercetator biolog Simona Stavri

Alina Penciu, asistent medical principal

Adriana Metcan, asistent medical

Mariana Popescu, psv I

Elisabeta Tomita, psv I

Sanda Stefan, ingrijitor

Obiective:

Activitatea din laborator vizeaza :

  • Expertiza microbiologica  prin tehnici de diagnostic clasic si molecular in domeniul infectiilor cu enterovirusuri si rotavirusuri.
  • Armonizarea metodelor de analiza moleculara a tulpinilor izolate in vederea participarii competitive la programele internationale de cercetare

Participare la programe internationale/ nationale:

  • Laboratorul este acreditat ca Laborator National de Referinta in cadrul Retelei  Globale a Laboratoarelor Polio  si participa la Programul OMS de Eradicare Globala a Poliomielitei
  • Programul Grupul de Studiu pentru Enterovirusuri din cadrul Retelei Internationale a Institutelor Pasteur  privind studierea variabilitatii genetice a tulpinilor de poliovirus si enterovirusuri nonpolio  izolate pe teritoriul Romanie  
  • Programul national de supraveghere a cazurilor de paralizie acuta flasca (PAF)
  • Programul national de supraveghere a meningitelor acute virale
  • Programul national de supraveghere a bolii diareice cu rotavirusuri

Determinari efectuate in cadrul laboratorului

  • Izolare si identificare enterovirusuri (poliovirusuri, coxsackievirusuri, ECHO virusuri) in probe recoltate de la pacienti cu paralizie acuta flasca,  poliradiculonevrite,  paralizii faciale, meningite virale acute, conjunctivite virale acute
  •  Titrare anticorpi serici neutralizanti  antipoliovirusuri
  •  Titrare anticorpi serici neutralizanti antienterovirusuri nepoliomielitice (pentru pacientii din ale caror produse patologice a fost izolat un enterovirus non polio)
  • Izolare si identificare enterovirusuri in probe recoltate din mediul inconjurator (namoluri si ape uzate)
  • Determinare rotavirusuri prin testul ELISA
  • Caracterizarea moleculara a tulpinilor de poliovirus si rotavirus izolate

Activitati suport:

  • Preparare de substraturi celulare, reactivi in house

Activitati didactice:   

  • Activitati de formare a specialistilor de medicina de laborator si epidemiologie,  tineri cercetatori, studenti
  • Publicatii metodologice 
  • Participare la  organizare de cursuri creditate de Colegiul Medicilor si Ordinul Biologilor si Biochimistilor 


Participarea la proiecte de cercetare interne si internationale perioada 2004-2011

Proiecte nationale coordonate

 2007-2010: CNCSIS Idei/ Nr.contract 286/2007 : Genotiparea tulinilor de poliovirus izolate in Romania in perioada 2007-2010 de la cazurile cu PAF si contactii sanatosi ai acestora ca obiectiv major in procesul de eradicare a poliomielitei. Director proiect Dr. Anda Baicus

Parteneri in proiecte nationale

2009-2011 : Proiect Nucleu:  Întãrirea si extinderea capacitãtii de supraveghere viralã în boli prevenibile prin vaccinare :rujeola,  rubeola,  oreion, poliomielita, difterie.

2008-2011:  PNII- Parteneriate/2008: Obtinerea de noi materiale antimicrobiene pentru controlul infectiilor    nosocomiale


2007-2010: PNII- Parteneriate/2007: Realizarea unui nou test tuberculinic cutanat pentru revelarea hipersensibilitatii de tip intarziat prin tehnologii avansate, cu utilizarea antigenelor recombinante specifice pentru diagnosticul tuberculozei latente si active


2006 -2008: CEEX: Diagnosticul si managementul sindromului diareic infectios

 

Parteneri in proiecte internationale

2008-2010 : Transverse Research Programs PTR 276: Co-circulation, interactions et evolution des enterovirus C et des poliovirus dans les pays du Reseau international des Instituts Pasteur (RIIP);  Coordonnateur Docteur Francis DELPEYROUX Unité Biologie des Virus Entériques, Institut Pasteur Paris, Coordonator INCDMI Cantacuzino Dr. Anda Baicus


2005-2007 : Proiect  international ACIP : Circulation des poliovirus et enterovirus non polio dans l’environnement


2005-2006: Proiect  international:  Molecular epidemiology of rotavirus in the Balkan/ Eastern European Areas  Istituto Superiore di Sanita, Rome, Italy 

 

2004-2006: Proiect  international ACIP :  Diagnostic moleculaire des enterovirus – Reteaua Internationala a Institutelor Pasteur

2004-2006: Proiect  international PTR: RT-PCR-RFLP utilise  dans l’etude de la circulation et la derive genetique  des souches  vaccinales des poliovirus – Reteaua Internationala a Institutelor Pasteur

Lucrari publicate – cotate ISI 

2007: Circulation of a type 1 recombinant vaccine-derived poliovirus strain in a limited area in Romania. Combiescu M, Guillot S, Persu A, Baicus A, Pitigoi D, Balanant J, Oprisan G, Crainic R, Delpeyroux F, Aubert-Combiescu A., Arch Virol. 2007;152(4):727-38. Epub 2007 Jan 3;  factor de impact: 2,02


Lucrari publicate in baze de date internationale

 2009: European Antibiotic Awareness Day -Collaborative  Group  European Antibiotic Awareness Day, 2008 – the first Europe-wide public information campaign on prudent antibiotic use: methods and survey of activities in participating countries. Earnshaw S, Monnet DL, Duncan B, O’Toole J, Ekdahl K, Goossens H; European Antibiotic Awareness Day Technical Advisory Committee; Euro Surveill., 2009, 30;14(30):19280.

European Antibiotic Awareness Day Collaborative Group:

Austria – H. Mittermayer (Antimicrobial Resistance National Focal Point – AMR NFP), R. Strauss (AMR NFP), S. Metz-Gercek; Belgium – H. Goossens (AMR NFP), S. Coenen; Bulgaria- T. Kantardjiev (AMR NFP), M. Petrov; Croatia – A. Tambic Andrasevic (AMR NFP); Cyprus – D. Pieridou-Bagatzouni (AMR NFP); Czech Republic – V. Jindrák (AMR NFP); Denmark – N.Frimodt-Mřller (AMR NFP), A.M. Hammerum; Estonia – K. Kutsar (AMR NFP); Finland – P.Huovinen (AMR NFP), A. Hakanen; France – J.-M. Azanowsky (AMR NFP), B. Schlemmer;Former Yugoslav Republic of Macedonia – G. Bosevska (AMR NFP); Germany – A. Barger (AMR NFP); Greece – H. Giamarellou, (AMR NFP), A. Antoniadou; Hungary – K. Böröcz (AMR NFP); Iceland – H. Briem (AMR NFP); Ireland – R. Cunney (AMR NFP); Italy – A. Pantosti (AMR NFP), P. Salcuni (AMR NFP); Latvia – S. Terela (AMR NFP), U. Dumpis; Lithuania – R. Valinteliene (AMR NFP); Luxembourg – E. Heisbourg (AMR NFP); Malta – M. Borg (AMR NFP), P. Zarb; Netherlands – J. Prins (AMR NFP), I.C. Gyssens, L. Wijgergangs; Norway, G.S. Simonsen (AMR NFP), G. Wřien, M. Lindbćk; Poland – W. Hryniewicz (AMR NFP), B. Mazinska, A. Olczak Pieńkowska; Portugal – A.C. Costa (AMR NFP), J. Melo Cristino; Romania – A.Băicuş (AMR NFP), A. Canton; Slovakia – L. Siegfried (AMR NFP), H. Hupkova; Slovenia – M. Čižman (AMR NFP); Spain – J. Campos (AMR NFP); Sweden – A. Tegnell (AMR NFP), I.Riesenfeld-Örn, O. Cars; Turkey – N. Çöplü (AMR NFP); United Kingdom – S. Wellsteed (AMR NFP), C. McNulty.


2009: A  Baicus, A Persu, M Popescu, A Penciu, S Stavri, A Soare,N Grecu, C Szmal, G Oprisan, Correlation between vaccine coverage against polio and circulation and genetic evolution of the poliovirus strains isolated in Romania in the framework of the global polio eradication strategy,Roum Arch Microbiol  Immunol.,2009, 4, 9-14.


2009: C Baicus, S Caraiola, A Baicus, R Tanasescu, M Rimbas, Involuntary weight loss: case series, etiology and diagnostic, Rom J Intern Med., 2009;47(1):87-92.


 2009: M Damian, D Tatu-Chitoiu, C R Usein, G Oprisan, AM Palade,S Dinu, C Szmal, S Ciontea, S Ceciu, M Condei, A Persu, A Baicus, M Pop, I Neagoe, DSteriu, R Codreanu, M Graur,M C Cretu, S Cilievici, MNica, A Ecovoiu,  L Gavrila, Laboratory diagnosis of infectious diarrhoea syndrome ; a three years study in two hospitals of infectious diseases Roum Arch. Microbiol Immunol, 2009, 2:89-94.


2009: A Persu , A Baicus, S Stavri, M.Combiescu.  Non-polio enteroviruses associated with acute flaccid paralysis (AFP) and facial paralysis (FP) cases in Romania, 2001-2008, Roum Arch Microbiol Immunol., 2009, 68(1):20-6.


2007: A Baicus, A  Persu ,M  Combiescu ,  A Aubert-Combiescu.  The maintaining of the active laboratory-based surveillance of the acute flaccid paralysis (AFP) cases in Romania in the framework of the strategic plan of the global polio eradication initiative, Roum Arch Microbiol Immunol., 2007, 66(1-2):44-50.

 

2006: A Baicus,  M Combiescu, A Persu, G Oprişan,  AAubert-Combiescu, F Delpeyroux. The molecular characterization of poliovirus strains by the RT-PCR-RFLP assay and its use in the active surveillance for acute flaccid paralysis cases in Romania between 2001-2006, Roum Arch Microbiol Immunol.2006, Jul-Dec; 65(3-4):120-30.


2006: A Baicus, A Persu, MI Popa, C Baicus. Immunity status against coxsackie viruses B in 25 patients with acute myocarditis, Roum Arch Microbiol Immunol.2006 Jul-Dec;65(3-4):109-12.


2006: A Baicus, M Combiescu, A Persu, A Aubert-Combiescu, Immune status to polioviruses in the child population of Romania between 2002-2005 as indicated by serological investigation in cases of acute flaccid paralysis (AFP) and facial paralysis (FP) and its use as a “national reference value” to evaluate the vaccination coverage in particular groups of healthy children, Rev Med Chir Soc Med Nat Iasi. 2006,   Oct-Dec;110(4):1004-11.